ナカムラ ノブヒロ   NAKAMURA NOBUHIRO
  中村 暢宏
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2008
形態種別 研究論文
査読 査読あり
標題 ERK regulates Golgi and centrosome orientation towards the leading edge through GRASP65
執筆形態 その他
掲載誌名 Journal of Cell Biology
出版社・発行元 ROCKEFELLER UNIV PRESS
巻・号・頁 182(5),pp.837-843
著者・共著者 Bisel, B.,Wang, Y.,Wei, J.-H.,Xiang, Y.,Tang, D.,Miron-Mendoza, M.,Yoshimura, S.-I.,Nakamura, N.,Seemann, J.
概要 Directed cell migration requires the orientation of the Golgi and centrosome toward the leading edge. We show that stimulation of interphase cells with the mitogens epidermal growth factor or lysophosphatidic acid activates the extracellular signal-regulated kinase (ERK), which phosphorylates the Golgi structural protein GRASP65 at serine 277. Expression of a GRASP65 Ser277 to alanine mutant or a GRASP65 1-201 truncation mutant, neither of which can be phosphorylated by ERK, prevents Golgi orientation to the leading edge in a wound assay. We show that phosphorylation of GRASP65 with recombinant ERK leads to the loss of GRASP65 oligomerization and causes Golgi cisternal unstacking. Furthermore, preventing Golgi polarization by expressing mutated GRASP65 inhibits centrosome orientation, which is rescued upon disassembly of the Golgi structure by brefeldin A. We conclude that Golgi remodeling, mediated by phosphorylation of GRASP65 by ERK, is critical for the establishment of cell polarity in migrating cells.
DOI 10.1083/jcb.200805045
ISSN 0021-9525
Put Code(ORCID) 19809397