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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Possible promotion of neuronal differentiation in fetal rat brain neural progenitor cells after sustained exposure to static magnetism |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Neuroscience Research |
| 出版社・発行元 | WILEY-LISS |
| 巻・号・頁 | 87(11),pp.2406-2417 |
| 著者・共著者 | Nakamichi, N.,Ishioka, Y.,Hirai, T.,Ozawa, S.,Tachibana, M.,Nakamura, N.,Takarada, T.,Yoneda, Y. |
| 概要 | We have previously shown significant potentiation of Ca(2+) influx mediated by N-methyl-D-aspartate receptors, along with decreased microtubules-associated protein-2 (MAP2) expression, in hippocampal neurons cultured under static magnetism without cell death. In this study, we investigated the effects of static magnetism on the functionality of neural progenitor cells endowed to proliferate for self-replication and differentiate into neuronal, astroglial, and oligodendroglial lineages. Neural progenitor cells were isolated from embryonic rat neocortex and hippocampus, followed by culture under static magnetism at 100 mT and subsequent determination of the number of cells immunoreactive for a marker protein of particular progeny lineages. Static magnetism not only significantly decreased proliferation of neural progenitor cells without affecting cell viability, but also promoted differentiation into cells immunoreactive for MAP2 with a concomitant decrease in that for an astroglial marker, irrespective of the presence of differentiation inducers. In neural progenitors cultured under static magnetism, a significant increase was seen in mRNA expression of several activator-type proneural genes, such as Mash 1, Math 1, and Math3, together with decreased mRNA expression of the repressor type Hes5. These results suggest that sustained static magnetism could suppress proliferation for self-renewal and facilitate differentiation into neurons through promoted expression of activator-type proneural genes by progenitor cells in fetal rat brain. (C) 2009 Wiley-Liss, Inc. |
| DOI | 10.1002/jnr.22087 |
| ISSN | 0360-4012 |
| Put Code(ORCID) | 19809393 |