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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2010 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Interaction of Golgin-84 with the COG Complex Mediates the Intra-Golgi Retrograde Transport |
| 執筆形態 | その他 |
| 掲載誌名 | Traffic |
| 出版社・発行元 | WILEY-BLACKWELL PUBLISHING, INC |
| 巻・号・頁 | 11(12),pp.1552-1566 |
| 著者・共著者 | Sohda, M.,Misumi, Y.,Yamamoto, A.,Nakamura, N.,Ogata, S.,Sakisaka, S.,Hirose, S.,Ikehara, Y.,Oda, K. |
| 概要 | The coiled-coil Golgi membrane protein golgin-84 functions as a tethering factor for coat protein I (COPI) vesicles. Protein interaction analyses have revealed that golgin-84 interacts with another tether, the conserved oligomeric Golgi (COG) complex, through its subunit Cog7. Therefore, we explored the function of golgin-84 as the tether for COPI vesicles of intra-Golgi retrograde traffic. First, glycosylic maturation of both plasma membrane (CD44) and lysosomal (lamp1) glycoproteins was distorted in golgin-84 knockdown (KD) cells. The depletion of golgin-84 caused fragmentation of the Golgi with the mislocalization of Golgi resident proteins, resulting in the accumulation of vesicles carrying intra-Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and cis-Golgi membrane protein GPP130. Similar observations were obtained by diminution of the COG complex, suggesting a strong correlation between the two tethers. Indeed, COG complex-dependent (CCD) vesicles that accumulate in Cog3 or Cog7 KD cells carried golgin-84. Surprisingly, the interaction between golgin-84 and another candidate tethering partner CASP (CDP/cut alternatively spliced product) decreased in Cog3 KD cells. These results indicate that golgin-84 on COPI vesicles interact with the COG complex before SNARE assembly, suggesting that the interaction of golgin-84 with COG plays an important role in the tethering process of intra-Golgi retrograde vesicle traffic. |
| DOI | 10.1111/j.1600-0854.2010.01123.x |
| ISSN | 1398-9219 |
| Put Code(ORCID) | 19809390 |