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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 発行・発表の年月 | 1994/10/28 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Isolation of the stable hexameric DnaK·DnaJ complex from Thermus thermophilus |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Biological Chemistry |
| 巻・号・頁 | 269(43),27074-27079頁 |
| 著者・共著者 | Motohashi K,Taguchi H,Ishii N,Yoshida M |
| 概要 | A DnaK homolog (T.DnaK) has been purified as a stable complex with a DnaJ homolog (T.DnaJ) from a thermophilic bacterium, Thermus thermophilus. This complex has an approximate molecular size of 300 kDa and appears to contain three copies of each of T.DnaK and T.DnaJ molecules. Consistently, trigonal ring structures with a diameter (trigonal apex-to-apex) of about 11 nm were observed with electron microscopy. The complex has no endogenously bound AT(D)P and is stable in the presence of Mg-AT(D)P. It possesses a weak ATPase activity and retains about 3 mol of ADP/mole of the complex when incubated with Mg-ATP. This complex is able to interact with the reduced carboxymethylated α-lactalbumin which we used as a model unfolded protein. |
| ISSN | 0021-9258 |
| PMID | 7929450 |
| Put Code(ORCID) | 21490293 |
| PermalinkURL | http://orcid.org/0000-0002-8414-2836 |