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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1997/08 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | K+ is an indispensable cofactor for GrpE stimulation of ATPase activity of DnaK center dot DnaJ complex from Thermus thermophilus |
| 執筆形態 | その他 |
| 掲載誌名 | FEBS LETTERS |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 412(3),pp.633-636 |
| 著者・共著者 | K Motohashi,M Yohda,M Odaka,M Yoshida |
| 概要 | K+ is an indispensable cofactor for ATPase activity of eukaryotic cytosolic Hsp70 chaperone systems which lack a GrpE homolog, In the case of the bacterial Hsp70 (DnaK) system, GrpE, a nucleotide exchange factor, stimulates ATPase activity but little is known about the effect of K+, Here, we have cloned a grpE gene from a thermophile, Thermus thermophilus, and purified a homodimeric GrpE protein. Using proteins of this bacterium, we found that the GrpE stimulation of ATPase activity of DnaK.DnaJ complex was absolutely dependent on the presence of K+. (C) 1997 Federation of European Biochemical Societies. |
| DOI | 10.1016/S0014-5793(97)00847-8 |
| ISSN | 0014-5793 |
| NAID | 80009798645 |
| PMID | 9276481 |