モトハシ タケシ   MOTOHASHI TAKESHI
  本橋 健
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2000/04
形態種別 研究論文
査読 査読あり
標題 Heat-inactivated proteins managed by DnaKJ-GrpE-ClpB chaperones are released as a chaperonin-recognizable non-native form
執筆形態 その他
掲載誌名 JOURNAL OF BIOLOGICAL CHEMISTRY
出版社・発行元 AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
巻・号・頁 275(17),pp.12388-12392
著者・共著者 YH Watanabe,K Motohashi,H Taguchi,M Yoshida
概要 Chaperones of Thermus thermophilus cooperate in reactivation of heat-inactivated proteins. The protein, inactivated at a high temperature in a TDnaKJ-GrpE set, recovered its activity during subsequent incubation with TClpB at moderate temperature (Motohashi, K., Watanabe, Y., Yohda, M., and Yoshida, M. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 7184-7189). Here, we report that the addition of chaperonin (Tcpn) at moderate temperature improves the yield of the TDnaKJ-GrpE-ClpB-dependent reactivation. The trap-Tcpn, which binds substrate protein but does not release it, inhibits reactivation severely. Maximum recovery is gained at substoichiometric amounts of each component of TDnaKJ, TGrpE, and TClpB relative to the substrate monomer, These observations indicate that, driven by ATP hydrolysis, TDnaRJ-GrpE-ClpB chaperones catalytically cooperate and release heat-inactivated protein as a non-native, chaperonin-recognizable folding intermediate.
DOI 10.1074/jbc.275.17.12388
ISSN 0021-9258
NAID 80011629119
PMID 10777521