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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2006/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | In vitro reconstitution of monogalactosyldiacylglycerol (MGDG) synthase regulation by thioredoxin |
| 執筆形態 | その他 |
| 掲載誌名 | FEBS LETTERS |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 580(17),pp.4086-4090 |
| 著者・共著者 | Yoshiki Yamaryo,Ken Motohashi,Ken-ichiro Takamiya,Toru Hisabori,Hiroyuki Ohta |
| 概要 | Monogalactosyldiacylglycerol (MGDG), a major membrane lipid of chloroplasts, is synthesized by MGDG synthase (MGD) localized in chloroplast envelope membranes. We investigated whether MGD activity is regulated in a redox-dependent manner using recombinant cucumber MGD overexpressed in Escherichia coli. We found that MGD activity is reversibly regulated by reduction and oxidation in vitro and that an intramolecular disulfide bond(s) is involved in MGD activation. Because thioredoxin efficiently reduced disulfide bonds to enhance MGD activity in vitro, MGD is potentially an envelope-bound thioredoxin target protein in higher plants. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| DOI | 10.1016/j.febslet.2006.06.050 |
| ISSN | 0014-5793 |
| NAID | 80019047462 |
| PMID | 16824521 |