モトハシ タケシ   MOTOHASHI TAKESHI
  本橋 健
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2013/06
形態種別 研究論文
査読 査読あり
標題 Systematic exploration of thioredoxin target proteins in plant mitochondria
執筆形態 その他
掲載誌名 Plant and Cell Physiology
巻・号・頁 54(6),pp.875-892
著者・共著者 Keisuke Yoshida,Ko Noguchi,Ken Motohashi,Toru Hisabori
概要 The thioredoxin (Trx) system is known to play a pivotal role in cellular redox regulation, but its target proteins in plant mitochondria remain largely uncharacterized. In this study, we systemically screened Trx target candidates in plant mitochondria. Mitochondrial protein extracts were prepared from Arabidopsis shoots, spinach leaves and potato tubers, and then subfractionated into soluble matrix and insoluble membrane fractions. Protein extracts were loaded onto an affinity column immobilizing Arabidopsis mitochondria-localized o-type Trx mutant protein, in which one of two internal cysteines at the active site was substituted by serine. Proteins forming mixed-disulfide intermediates with the mutated Trx were identified by proteomic approaches. This procedure allowed the determination of 101 Trx target candidate proteins involved in a broad spectrum of mitochondrial processes. Furthermore, biochemical assay revealed that one of the potential Trx target proteins, alternative oxidase, is actually redox regulated by Trx. This study provides insights into the regulatory mechanism of diverse functions in mitochondrial biology that are mediated through the Trx system. © 2013 The Author.
DOI 10.1093/pcp/pct037
ISSN 0032-0781
PMID 23444301
Put Code(ORCID) 21490255
PermalinkURL http://www.scopus.com/inward/record.url?eid=2-s2.0-84878846165&partnerID=MN8TOARS
researchmap用URL http://orcid.org/0000-0002-8414-2836