|
モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2014/09 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Method for enhancement of plant redox-related protein expression and its application for in vitro reduction of chloroplastic thioredoxins |
| 執筆形態 | その他 |
| 掲載誌名 | PROTEIN EXPRESSION AND PURIFICATION |
| 出版社・発行元 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 101,pp.152-156 |
| 著者・共著者 | Ken Motohashi,Yuki Okegawa |
| 概要 | Plant redox-related proteins were overexpressed using a genetic codon substitution downstream of the translation initiation codon. This method significantly improved recombinant protein expression levels of Arabidopsis chloroplastic thioredoxins and cytosolic nicotinamide adenine dinucleotide phosphate (NADPH)-dependent thioredoxin reductase (E.C. 1.8.1.9) in Escherichia coli. Using these proteins, the in vitro chloroplastic thioredoxins-reduction system was reconstituted in an NADPH-dependent manner. This system could convert the five classes of chloroplastic Arabidopsis thioredoxins and two chloroplastic Spinach thioredoxins to their reduced forms, independent of dithiothreitol and the photosynthetic electron transport system. (C) 2014 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.pep.2014.07.001 |
| ISSN | 1046-5928/1096-0279 |
| Put Code(ORCID) | 21490254 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84905247175&partnerID=MN8TOARS |
| researchmap用URL | http://orcid.org/0000-0002-8414-2836 |