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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2015 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Evaluation of seamless ligation cloning extract preparation methods from an Escherichia coli laboratory strain |
| 執筆形態 | その他 |
| 掲載誌名 | Analytical Biochemistry |
| 出版社・発行元 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 486,pp.51-53 |
| 著者・共著者 | Okegawa, Y.,Motohashi, K. |
| 概要 | Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA cloning without the use of restriction enzymes. Instead, SLiCE uses homologous recombination activities from Escherichia coil cell lysates. To date, SLiCE preparation has been performed using an expensive commercially available lytic reagent. To expand the utility of the SLiCE method, we evaluated different methods for SLiCE preparation that avoid using this reagent. Consequently, cell extracts prepared with buffers containing Triton X-100, which is a common and low-cost nonionic detergent, exhibited sufficient cloning activity for seamless gene incorporation into a vector. (C) 2015 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.ab.2015.06.031 |
| ISSN | 0003-2697/1096-0309 |
| Put Code(ORCID) | 21490295 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84937865066&partnerID=MN8TOARS |