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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2018/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Comparative transcriptomics with self-organizing map reveals cryptic photosynthetic differences between two accessions of North American Lake cress |
| 執筆形態 | その他 |
| 掲載誌名 | Scientific Reports |
| 出版社・発行元 | Springer Nature |
| 巻・号・頁 | 8(1),pp.3302 |
| 著者・共著者 | Hokuto Nakayama,Tomoaki Sakamoto,Yuki Okegawa,Kaori Kaminoyama,Manabu Fujie,Yasunori Ichihashi,Tetsuya Kurata,Ken Motohashi,Ihsan Al-Shehbaz,Neelima Sinha,Seisuke Kimura |
| 概要 | Because natural variation in wild species is likely the result of local adaptation, it provides a valuable resource for understanding plant-environmental interactions. Rorippa aquatica (Brassicaceae) is a semi-aquatic North American plant with morphological differences between several accessions, but little information available on any physiological differences. Here, we surveyed the transcriptomes of two R. aquatica accessions and identified cryptic physiological differences between them. We first reconstructed a Rorippa phylogeny to confirm relationships between the accessions. We performed large-scale RNA-seq and de novo assembly
the resulting 87,754 unigenes were then annotated via comparisons to different databases. Between-accession physiological variation was identified with transcriptomes from both accessions. Transcriptome data were analyzed with principal component analysis and self-organizing map. Results of analyses suggested that photosynthetic capability differs between the accessions. Indeed, physiological experiments revealed between-accession variation in electron transport rate and the redox state of the plastoquinone pool. These results indicated that one accession may have adapted to differences in temperature or length of the growing season. |
| DOI | 10.1038/s41598-018-21646-w |
| ISSN | 2045-2322 |
| PMID | 29459626 |
| Put Code(ORCID) | 41613566 |
| PermalinkURL | http://orcid.org/0000-0002-8414-2836 |