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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 発行・発表の年月 | 2019/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products |
| 執筆形態 | その他 |
| 掲載誌名 | Scientific Reports |
| 出版社・発行元 | Springer Science and Business Media {LLC} |
| 巻・号・頁 | 9(1),6417頁 |
| 著者・共著者 | Ken Motohashi |
| 概要 | © 2019, The Author(s). An efficient PCR cloning method is indispensable in modern molecular biology, as it can greatly improve the efficiency of DNA cloning processes. Here, I describe the development of three vectors for TA cloning and blunt-end cloning. Specifically, pCRT and pCRZeroT were designed to improve the efficiency of TA cloning. pCRZeroT can also be used with pCRZero to facilitate blunt-end cloning using the ccdB gene. Using pCRZero and pCRZeroT and applying the Golden Gate reaction, I developed a direct PCR cloning protocol with non-digested circular vectors and PCR products. This direct PCR cloning protocol yielded colony-formation rates and cloning efficiencies that are comparable with those obtained by conventional PCR cloning with pre-digested vectors and PCR products. The three plasmids I designed are available from Addgene (https://www.addgene.org/). |
| DOI | 10.1038/s41598-019-42868-6 |
| PMID | 31015513 |
| Put Code(ORCID) | 56696379 |
| PermalinkURL | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064912955&origin=inward |