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チバ シノブ
CHIBA SHINOBU
千葉 志信 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2006/04 |
| 形態種別 | その他 |
| 標題 | The Escherichia coli plasma membrane contains two PHB (prohibitin homology) domain protein complexes of opposite orientations |
| 執筆形態 | その他 |
| 掲載誌名 | MOLECULAR MICROBIOLOGY |
| 出版社・発行元 | BLACKWELL PUBLISHING |
| 巻・号・頁 | 60(2),pp.448-457 |
| 著者・共著者 | S Chiba,K Ito,Y Akiyama |
| 概要 | Two membrane proteases, FtsH and HtpX, are jointly essential for Escherichia coli cell viability, presumably through their abilities to degrade abnormal membrane proteins. To search for additional cellular factors involved in membrane protein quality control, we isolated multicopy suppressors that alleviated the growth defect of the ftsH/htpX dual disruption mutant. One of them was ybbK, which is renamed qmcA, encoding a membrane-bound prohibitin homology (PHB) domain family protein. Multicopy suppression was also observed with hflK-hflC, encoding another set of PHB domain membrane proteins, which had been known to form a complex (HflKC) and to interact with FtsH. Whereas the Delta ftsH sfhC21 (a viability defect suppressor for Delta ftsH) strain exhibited temperature sensitivity in the presence of cAMP, additional disruption of both qmcA and hflK-hflC exaggerated the growth defect. Pull-down and sedimentation experiments showed that QmcA, like HflKC, forms an oligomer and interacts with FtsH. Protease accessibility assays revealed that QmcA, unlike periplasmically exposed HflKC, possesses a cytoplasmically disposed large C-terminal domain, thus assuming the type I (N-OUT-C-IN) orientation. We discuss possible significance of having PHB domains on both sides of the membrane. |
| DOI | 10.1111/j.1365-2958.2006.05104.x |
| ISSN | 0950-382X |