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カワネ コウキ
KAWANE KOKI
川根 公樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003/02 |
| 形態種別 | その他 |
| 標題 | Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation |
| 執筆形態 | その他 |
| 掲載誌名 | NATURE IMMUNOLOGY |
| 出版社・発行元 | NATURE AMERICA INC |
| 巻・号・頁 | 4(2),pp.138-144 |
| 著者・共著者 | K Kawane, H Fukuyama, H Yoshida, H Nagase, Y Ohsawa, Y Uchiyama, K Okada, T Iida, S Nagata |
| 概要 | Apoptosis is often accompanied by the degradation of chromosomal DNA. Caspase-activated DNase (CAD) is an endonuclease that is activated in dying cells, whereas DNase II is present in the lysosomes of macrophages. Here, we show that CAD(-/-) thymocytes did not undergo apoptotic DNA degradation. But, when apoptotic cells were phagocytosed by macrophages, their DNA was degraded by DNase II. The thymus of DNase II-/- CAD(-/-) embryos contained many foci carrying undigested DNA and the cellularity was severely reduced due to a block in T cell development. The interferon-beta gene was strongly up-regulated in the thymus of DNase II-/- CAD(-/-) embryos, suggesting that when the DNA of apoptotic cells is left undigested, it can activate innate immunity leading to defects in thymic development. |
| DOI | 10.1038/ni881 |
| ISSN | 1529-2908 |