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カワネ コウキ
KAWANE KOKI
川根 公樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1999/08 |
| 形態種別 | その他 |
| 標題 | Structure and promoter analysis of murine CAD and ICAD genes |
| 執筆形態 | その他 |
| 掲載誌名 | CELL DEATH AND DIFFERENTIATION |
| 出版社・発行元 | STOCKTON PRESS |
| 巻・号・頁 | 6(8),pp.745-752 |
| 著者・共著者 | K Kawane, H Fukuyama, M Adachi, H Sakahira, NG Copeland, DJ Gilbert, NA Jenkin, S Nagata |
| 概要 | Caspase-activated DNase (CAD) degrades chromosomal DNA during apoptosis, whereas ICAD (inhibitor of CAD) inhibits the CAD's DNase by binding to it. Here, we describe the assignment of murine CAD and ICAD genes to the distal part of murine chromosome 4, Molecular cloning and structural analysis indicated that CAD and ICAD genes are comprised of 7 and 6 exons, respectively. Two different ICAD mRNAs coding for two forms of ICAD proteins (ICAD-S and ICAD-L) were found to be produced by alternative splicing of intron 5. The CAD and ICAD mRNAs were detected ubiquitously in various murine tissues. Analyses of the promoter activity with a series of deletion mutants of their 5' flanking regions indicated that a 190-bp 5' flanking region of the CAD gene was sufficient to promote the transcription. Whereas, a 120-bp flanking region of ICAD gene was required to promote its transcription. These regions do not show similarity between CAD and ICAD genes, suggesting that expression of CAD and ICAD genes is regulated by different mechanisms. |
| ISSN | 1350-9047 |