|
川上 雅弘 所属 京都産業大学 生命科学部 産業生命科学科 職種 准教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2002 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes |
| 執筆形態 | その他 |
| 掲載誌名 | Biology of Reproduction |
| 掲載区分 | 国外 |
| 出版社・発行元 | SOC STUDY REPRODUCTION |
| 巻・号・頁 | 67(2),pp.442-446 |
| 著者・共著者 | Xi Jun Yin,Tetsuya Tani,Isao Yonemura,Masahiro Kawakami,Kazunori Miyamoto,Rie Hasegawa,Yoko Kato,Yukio Tsunoda |
| 概要 | The present study demonstrated that brief treatment of in vitro-matured porcine oocytes with demecolcine results in a membrane protrusion that contains a condensed chromosome mass, which can be easily removed by aspiration. This simple, chemically assisted method for removing maternal chromosomes enabled the production of a large number of nuclear-transferred porcine eggs. The development of eggs whose chromosomes were removed by this procedure following transfer of somatic cell nuclei to the blastocyst stage was not significantly different among groups activated using different procedures (6% to 11%) and was also not different among donor cells of different origins (3% to 9%), except for cumulus cells (0.4%). After transfer of 180 to 341 nuclear-transferred eggs that received somatic cells to 6 recipients, 2 of the recipients produced 8 healthy cloned piglets from the heart cells of a female pig. The chemically assisted method for removing maternal chromosomes was also effective for bovine and rabbit eggs. |
| DOI | 10.1095/biolreprod67.2.442 |
| ISSN | 0006-3363 |
| PMID | 12135879 |
| PermalinkURL | http://www.biolreprod.org/content/67/2/442.long |