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川上 雅弘 所属 京都産業大学 生命科学部 産業生命科学科 職種 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Effect of Demecolcine and Nocodazole on the Efficiency of Chemically Assisted Removal of Chromosomes and the Developmental Potential of Nuclear Transferred Porcine Oocytes |
| 執筆形態 | その他 |
| 掲載誌名 | Cloning and Stem Cells |
| 掲載区分 | 国外 |
| 出版社・発行元 | Mary Ann Liebert Inc. |
| 巻・号・頁 | 5(4),pp.379-387 |
| 著者・共著者 | Masahiro Kawakami,Tetsuya Tani,Akiko Yabuuchi,Tatsuya Kobayashi,Hiroshi Murakami,Tatsuya Fujimura,Yoko Kato,Yukio Tsunoda |
| 概要 | Brief treatment of metaphase II (MII) stage porcine oocytes with 0.4 μg/mL demecolcine in the presence of 0.05 M sucrose produces a membrane protrusion that contains a condensed chromosome mass. The present study examined the optimal conditions for demecolcine and nocodazole treatment in chemically assisted removal of chromosomes. When matured oocytes were treated with 0.1-0.4 μg/mL demecolcine for 60 min or with 0.4 μg/mL demecolcine for 30 min or 3 μrg/mL nocodazole for 30 or 60 min, more than 70% of oocytes had a membrane protrusion containing condensed chromosomes were located. There was no difference in the in vitro developmental potential of enucleated oocytes assisted by 0.1 and 0.4 μg/mL demecolcine or 3 μrg/mL nocodazole that received porcine somatic cells. After transfer to 10 recipients, however, two of six recipients that received demecolcine-treated enucleated eggs produced four healthy cloned piglets, but none of the four recipients of nocodazole-treated enucleated eggs produced piglets. Further studies are required to increase the successful development to term because the proportion of live piglets was low (4/2, 672, 0.15%). |
| DOI | 10.1089/153623003772032871 |
| ISSN | 1536-2302 |
| PMID | 14733755 |