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川上 雅弘 所属 京都産業大学 生命科学部 産業生命科学科 職種 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The effects of time of first cleavage, developmental stage, and delipidation of nuclear-transferred porcine blastocysts on survival following vitrification |
| 執筆形態 | その他 |
| 掲載誌名 | Animal Reproduction Science |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 106(3-4),pp.402-411 |
| 著者・共著者 | M. Kawakami,Y. Kato,Y. Tsunoda |
| 概要 | The effect of removing cytoplasmic lipid droplets (delipidation) at the 2-cell and developmental stages on the survival of porcine somatic cell nuclear-transferred blastocysts developed from the enucleated oocytes receiving somatic cells from kidney of an adult female after cryopreservation was examined. Vitrification was performed using the Cryoloop method with a small volume of medium (0.5 μl). To select 2-cell embryos with a high potential to develop into blastocysts, the relationship between the timing of the first cleavage and the developmental potential was examined. The potential of nuclear-transferred oocytes to develop into blastocysts in the intermediate-cleavage group (20-24 h after activation, 25%) was slightly or significantly (P < 0.05) higher than that in fast-cleavage (<20 h after activation, 13%) and slow-cleavage groups (>24 h after activation, 5%). Most non-delipidated blastocysts did not survive after thawing (0% for early-stage and 9% for advanced-stage blastocysts), but the survival rate of delipidated blastocysts 48 h after culture (54% and 72%, respectively) was not significantly different from that of non-vitrified blastocysts (80% and 92%, respectively). The survival rate of advanced-stage blastocysts after vitrification was slightly higher than that of early-stage blastocysts. The present study demonstrates that somatic cell nuclear-transferred porcine blastocysts developed from embryos selected at the 2-cell stage can be preserved by vitrification with a small volume of medium if the lipid droplets of the embryos are first removed. © 2007. |
| DOI | 10.1016/j.anireprosci.2007.06.002 |
| ISSN | 0378-4320 |
| PMID | 17628361 |
| PermalinkURL | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T43-4NYJ0S8-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=2efee339fb6d67429b4b1827507fc07b |