ウシオダ リョウ   USHIODA RYO
  潮田 亮
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   准教授
発行・発表の年月 2021/12
形態種別 研究論文
標題 Ca2+ imbalance caused by ERdj5 deletion affects mitochondrial fragmentation
執筆形態 その他
掲載誌名 Scientific Reports
出版社・発行元 Springer Science and Business Media LLC
巻・号・頁 11(1)
著者・共著者 Riyuji Yamashita,Shohei Fujii,Ryo Ushioda,Kazuhiro Nagata
概要 <title>Abstract</title>The endoplasmic reticulum (ER) is the organelle responsible for the folding of secretory/membrane proteins and acts as a dynamic calcium ion (Ca2+) store involved in various cellular signalling pathways. Previously, we reported that the ER-resident disulfide reductase ERdj5 is involved in the ER-associated degradation (ERAD) of misfolded proteins in the ER and the activation of SERCA2b, a Ca2+ pump on the ER membrane. These results highlighted the importance of the regulation of redox activity in both Ca2+ and protein homeostasis in the ER. Here, we show that the deletion of ERdj5 causes an imbalance in intracellular Ca2+ homeostasis, the activation of Drp1, a cytosolic GTPase involved in mitochondrial fission, and finally the aberrant fragmentation of mitochondria, which affects cell viability as well as phenotype with features of cellular senescence. Thus, ERdj5-mediated regulation of intracellular Ca2+ is essential for the maintenance of mitochondrial homeostasis involved in cellular senescence.
DOI 10.1038/s41598-021-99980-9
ISSN /2045-2322
PermalinkURL https://www.nature.com/articles/s41598-021-99980-9.pdf
researchmap用URL https://www.nature.com/articles/s41598-021-99980-9