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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2017/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish |
| 執筆形態 | その他 |
| 掲載誌名 | GENES TO CELLS |
| 出版社・発行元 | WILEY |
| 巻・号・頁 | 22(7),pp.670-678 |
| 担当区分 | 筆頭著者,責任著者 |
| 著者・共著者 | Yuichiro Mishima,Yukihide Tomari |
| 概要 | mRNA degradation is a fundamental biological process that erases transcribed genetic information from cells. During maternal-to-zygotic transition of animal development, thousands of maternal mRNAs are degraded by multiple mechanisms including microRNAs and codon-mediated decay. Enzymatic requirements for maternal mRNA clearance, however, are not fully understood. Here, we analyzed a contribution of the decapping enzyme Dcp2 to maternal mRNA clearance in zebrafish by over-expressing catalytically inactive Dcp2 and performing RNA-seq analysis. As expected, Dcp2 had a widespread role in maternal mRNA clearance. Interestingly, each mRNA showed differential dependency on Dcp2-mediated decapping and Cnot7-mediated deadenylation for degradation. Correlation analysis identified several mRNA features that were associated with the observed differential dependency. Our results show pervasive yet nonuniform contributions of the decapping enzyme Dcp2 and the deadenylase Cnot7 to maternal mRNA clearance. |
| DOI | 10.1111/gtc.12504 |
| ISSN | 1356-9597/1365-2443 |
| PMID | 28557307 |