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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2020/05/05 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Genome-wide Survey of Ribosome Collision. |
| 執筆形態 | その他 |
| 掲載誌名 | Cell reports |
| 掲載区分 | 国外 |
| 巻・号・頁 | 31(5),pp.107610-107610 |
| 著者・共著者 | Peixun Han,Yuichi Shichino,Tilman Schneider-Poetsch,Mari Mito,Satoshi Hashimoto,Tsuyoshi Udagawa,Kenji Kohno,Minoru Yoshida,Yuichiro Mishima,Toshifumi Inada,Shintaro Iwasaki |
| 概要 | Ribosome movement is not always smooth and is rather often impeded. For ribosome pauses, fundamental issues remain to be addressed, including where ribosomes pause on mRNAs, what kind of RNA/amino acid sequence causes this pause, and the physiological significance of this attenuation of protein synthesis. Here, we survey the positions of ribosome collisions caused by ribosome pauses in humans and zebrafish using modified ribosome profiling. Collided ribosomes, i.e., disomes, emerge at various sites: Pro-Pro/Gly/Asp motifs; Arg-X-Lys motifs; stop codons; and 3' untranslated regions. The electrostatic interaction between the charged nascent chain and the ribosome exit tunnel determines the eIF5A-mediated disome rescue at the Pro-Pro sites. In particular, XBP1u, a precursor of endoplasmic reticulum (ER)-stress-responsive transcription factor, shows striking queues of collided ribosomes and thus acts as a degradation substrate by ribosome-associated quality control. Our results provide insight into the causes and consequences of ribosome pause by dissecting collided ribosomes. |
| DOI | 10.1016/j.celrep.2020.107610 |
| PMID | 32375038 |