ミシマ ユウイチロウ   Mishima Yuichiro
  三嶋 雄一郎
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   准教授
言語種別 英語
発行・発表の年月 2020/12
形態種別 研究論文
査読 査読あり
標題 Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
執筆形態 その他
掲載誌名 STAR Protocols
掲載区分国外
出版社・発行元 Elsevier BV
巻・号・頁 1(3),pp.100168-100168
著者・共著者 Mari Mito,Yuichiro Mishima,Shintaro Iwasaki
概要 Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows the survey of sites in a genome-wide manner. This protocol details the procedure from lysate preparation of human tissue cultures and zebrafish embryos to sequencing library construction. For complete details on the use and execution of this protocol, please refer to Han et al. (2020).
DOI 10.1016/j.xpro.2020.100168
ISSN 2666-1667
PMID 33377062