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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2006/11 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430 |
| 執筆形態 | その他 |
| 掲載誌名 | CURRENT BIOLOGY |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 16(21),pp.2135-2142 |
| 担当区分 | 筆頭著者 |
| 著者・共著者 | Yuichiro Mishima,Antonio J. Giraldez,Yasuaki Takeda,Toshinobu Fujiwara,Hiroshi Sakamoto,Alexander F. Schier,Kunio Inoue |
| 概要 | Early in development, primordial germ cells (PGCs) are set aside from somatic cells and acquire a unique gene-expression program [1].The mechanisms underlying germline-specific gene expression are largely unknown. Nanos expression is required during germline development [2-5] and is posttranscriptionally restricted to PGCs [4,6-8]. Here we report that the microRNA miR-430 targets the 3' untranslated region (UTR) of nanos1 during zebrafish embryogenesis. A miR430 target site within the nanos1 3' UTR reduces poly(A) tail length, mRNA stability, and translation. Repression is disrupted in maternal-zygotic dicer mutants (MZdicer), which lack mature miRNAs [9], and is restored by injection of processed miR-430. Although miR-430 represses other genes equally in germline and soma, specific regions in the nanos1 31 UTR compensate for microRNA-mediated repression in PGCs and allow germline-specific expression. We show that the 3' UTR of an additional PGC-specific gene, TDRD7, is also targeted by miR-430. These results indicate that miR-430 targets the 3' UTRs of germline genes and suggest that differential susceptibility to microRNAs contributes to tissue-specific gene expression. |
| DOI | 10.1016/j.cub.2006.08.086 |
| ISSN | 0960-9822 |
| PMID | 17084698 |