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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2022/01/18 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Ribosome slowdown triggers codon-mediated mRNA decay independently of ribosome quality control. |
| 執筆形態 | その他 |
| 掲載誌名 | The EMBO journal |
| 掲載区分 | 国外 |
| 巻・号・頁 | pp.e109256 |
| 担当区分 | 筆頭著者,責任著者 |
| 著者・共著者 | Yuichiro Mishima,Peixun Han,Kota Ishibashi,Seisuke Kimura,Shintaro Iwasaki |
| 概要 | The control of mRNA stability plays a central role in regulating gene expression patterns. Recent studies have revealed that codon composition in the open reading frame determines mRNA stability in multiple organisms. Based on genome-wide correlation approaches, this previously unrecognized role for the genetic code is attributable to the kinetics of the codon-decoding process by the ribosome. However, complementary experimental analyses are required to clarify the codon effects on mRNA stability and the related cotranslational mRNA decay pathways, for example, those triggered by aberrant ribosome stalling. In the current study, we performed a set of reporter-based analyses to define codon-mediated mRNA decay and ribosome stall-dependent mRNA decay in zebrafish embryos. Our analysis showed that the effect of codons on mRNA stability stems from the decoding process, independent of the ribosome quality control factor Znf598 and stalling-dependent mRNA decay. We propose that codon-mediated mRNA decay is rather triggered by transiently slowed ribosomes engaging in a productive translation cycle in zebrafish embryos. |
| DOI | 10.15252/embj.2021109256 |
| PMID | 35040509 |