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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish |
| 執筆形態 | その他 |
| 掲載誌名 | DEVELOPMENT |
| 出版社・発行元 | COMPANY OF BIOLOGISTS LTD |
| 巻・号・頁 | 139(23),pp.4356-4364 |
| 担当区分 | 責任著者 |
| 著者・共著者 | Carlos Stahlhut,Yajaira Suarez,Jun Lu,Yuichiro Mishima,Antonio J. Giraldez |
| 概要 | Cellular communication across tissues is an essential process during embryonic development. Secreted factors with potent morphogenetic activity are key elements of this cross-talk, and precise regulation of their expression is required to elicit appropriate physiological responses. MicroRNAs (miRNAs) are versatile post-transcriptional modulators of gene expression. However, the large number of putative targets for each miRNA hinders the identification of physiologically relevant miRNA-target interactions. Here we show that miR-1 and miR-206 negatively regulate angiogenesis during zebrafish development. Using target protectors, our results indicate that miR-1/206 directly regulate the levels of Vascular endothelial growth factor A (VegfA) in muscle, controlling the strength of angiogenic signaling to the endothelium. Conversely, reducing the levels of VegfAa, but not VegfAb, rescued the increase in angiogenesis observed when miR-1/206 were knocked down. These findings uncover a novel function for miR-1/206 in the control of developmental angiogenesis through the regulation of VegfA, and identify a key role for miRNAs as regulators of cross-tissue signaling. |
| DOI | 10.1242/dev.083774 |
| ISSN | 0950-1991 |
| PMID | 23132244 |