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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1993/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | NUCLEOTIDE-SEQUENCE OF A GENOMIC GENE ENCODING TRITIN, A RIBOSOME-INACTIVATING PROTEIN FROM TRITICUM-AESTIVUM |
| 執筆形態 | その他 |
| 掲載誌名 | PLANT MOLECULAR BIOLOGY |
| 出版社・発行元 | KLUWER ACADEMIC PUBL |
| 巻・号・頁 | 22(1),pp.171-176 |
| 著者・共著者 | N HABUKA,J KATAOKA,M MIYANO,H TSUGE,H AGO,M NOMA |
| 概要 | A genomic gene of tritin, a ribosome-inactivating protein (RIP) from Triticum aestivum, was cloned using a barley RIP gene as a probe. The 5'-non-coding region has potential TATA boxes and three sequences homologous to the binding sequence of the transcriptional activator protein Opaque-2 which activates maize RIP gene expression. The cloned DNA encoded tritin consists of 275 amino acids with no secretion signal sequence. The coding region of tritin was expressed in Escherichia coli using lac promoter and yielded a protein similar to the native one, as determined by SDS-polyacrulamide gel electrophoresis and immunological analysis. |
| ISSN | 0167-4412 |