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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2020/01/24 |
| 形態種別 | その他 |
| 査読 | 査読あり |
| 標題 | ERdj3B-mediated quality control maintains anther development at high temperatures. |
| 執筆形態 | その他 |
| 掲載誌名 | Plant physiology |
| 掲載区分 | 国外 |
| 著者・共著者 | Masaya Yamamoto,Shuhei Uji,Tomoyuki Sugiyama,Tomoaki Sakamoto,Seisuke Kimura,Toshiya Endo,Shuh-Ichi Nishikawa |
| 概要 | Pollen development is highly sensitive to heat stress, which impairs cellular proteostasis by causing misfolded proteins to accumulate. Therefore, each cellular compartment possesses a dedicated protein quality control system. An elaborate quality control system involving molecular chaperones, including immunoglobulin binding protein (BiP), heat shock protein 70, and regulatory J domain-containing co-chaperones (J proteins), allows the endoplasmic reticulum (ER) to withstand a large influx of proteins. Here, we found that Arabidopsis thaliana mutants of ER-localized DnaJ family 3B (ERdj3B), one of three ER-resident J proteins involved in ER quality control, produced few seeds at high temperatures (29{degree sign}C) due to defects in anther development. This temperature-sensitive fertility defect is specific to the defective interactions of BiP with ERdj3B, but not with the other two J proteins, indicating functional differences between ERdj3B and the other J proteins. RNA-seq analysis revealed that heat stress affects pollen development in both wild-type and mutant buds, but the erdj3b mutant is more susceptible, possibly due to defects in ER quality control. Our results highlight the importance of a specific ER quality control factor, ERdj3B, for plant reproduction, particularly anther development, at high temperatures. |
| DOI | 10.1104/pp.19.01356 |
| PMID | 31980572 |