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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2013/08 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | In Vivo Fluorescent Adenosine 5'-Triphosphate (ATP) Imaging of Drosophila melanogaster and Caenorhabditis elegans by Using a Genetically Encoded Fluorescent ATP Biosensor Optimized for Low Temperatures |
| 執筆形態 | その他 |
| 掲載誌名 | ANALYTICAL CHEMISTRY |
| 出版社・発行元 | AMER CHEMICAL SOC |
| 巻・号・頁 | 85(16),pp.7889-7896 |
| 著者・共著者 | Taiichi Tsuyama,Jun-ichi Kishikawa,Yong-Woon Han,Yoshie Harada,Asako Tsubouchi,Hiroyuki Noji,Akira Kakizuka,Ken Yokoyama,Tadashi Uemura,Hiromi Imamura |
| 概要 | Adenosine 5'-triphosphate (ATP) is the major energy currency of all living organisms. Despite its important 4 functions, the spatiotemporal dynamics of ATP levels inside 3 living multicellular organisms is unclear. In this study, we 2 modified the genetically encoded Forster resonance energy transfer (FRET)-based ATP biosensor ATeam to optimize its affinity at low temperatures. This new biosensor, AT1.03NL, detected ATP changes inside Drosophila S2 cells more sensitively than the original biosensor did, at 25 degrees C. By expressing AT1.03NL in Drosophila melanogaster and Caenorhabditis elegans, we succeeded in imaging the in vivo ATP dynamics of these model animals at single-cell resolution. |
| DOI | 10.1021/ac4015325 |
| ISSN | 0003-2700 |
| PMID | 23875533 |