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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Codon Usage and 30 UTR Length Determine Maternal mRNA Stability in Zebrafish |
| 執筆形態 | その他 |
| 掲載誌名 | MOLECULAR CELL |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 61(6),pp.874-885 |
| 担当区分 | 筆頭著者,責任著者 |
| 著者・共著者 | Yuichiro Mishima,Yukihide Tomari |
| 概要 | The control of mRNA stability plays a central role in regulating gene expression. In metazoans, the earliest stages of development are driven by maternally supplied mRNAs. The degradation of these maternal mRNAs is critical for promoting the maternal-to-zygotic transition of developmental programs, although the underlying mechanisms are poorly understood in vertebrates. Here, we characterized maternal mRNA degradation pathways in zebrafish using a transcriptome analysis and systematic reporter assays. Our data demonstrate that ORFs enriched with uncommon codons promote deadenylation by the CCR4-NOT complex in a translationdependent manner. This codon-mediated mRNA decay is conditional on the context of the 30 UTR, with long 30 UTRs conferring resistance to deadenylation. These results indicate that the combined effect of codon usage and 30 UTR length determines the stability of maternal mRNAs in zebrafish embryos. Our study thus highlights the codon-mediated mRNA decay as a conserved regulatory mechanism in eukaryotes. |
| DOI | 10.1016/j.molcel.2016.02.027 |
| ISSN | 1097-2765/1097-4164 |
| PMID | 26990990 |