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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1999/06 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Crystallization and preliminary X-ray studies of the Ia component of Clostridium perfringens iota toxin complexed with NADPH |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF STRUCTURAL BIOLOGY |
| 出版社・発行元 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 126(2),pp.175-177 |
| 担当区分 | 筆頭著者,責任著者 |
| 著者・共著者 | H Tsuge,M Nagahama,T Nishimura,Y Sakaguchi,N Katunuma,J Sakurai |
| 概要 | A recombinant Ia component of Clostridium perfringens iota toxin, which ADP-ribosylates actin, was crystallized by the hanging drop vapor diffusion method using PEG4000 as a precipitating agent. The crystals were obtained in the presence of NADPH, which is similar to a real substrate, NADH, and belongs to the space group P1 (a = 47.9 Angstrom b = 54.5 Angstrom, c = 103.1 Angstrom, alpha = 99.0 degrees, beta = 93.3 degrees, and gamma = 107.2 degrees). The Matthews coefficient of native crystal was 2.7, assuming 2 mol/asymmetric unit. Native data were collected at 2.4-Angstrom resolution. The results from a heavy-atom search showed that lanthanide ions (samarium, holmium) altered the molecular packing, judging from the unit-cell difference. The crystals also belonged to the space group P1 (a = 47.7 Angstrom, b = 53.9 Angstrom, c = 54.6 Angstrom, alpha = 68.9 degrees, beta = 78.3 degrees, and gamma = 73.7 degrees), which is consistent with only one molecule per asymmetric unit. (C) 1999 Academic Press. |
| DOI | 10.1006/jsbi.1999.4124 |
| ISSN | 1047-8477/1095-8657 |