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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/06 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF CELL BIOLOGY |
| 出版社・発行元 | ROCKEFELLER UNIV PRESS |
| 巻・号・頁 | 185(6),pp.1029-1045 |
| 著者・共著者 | Yasushi Tamura,Toshiya Endo,Miho Iijima,Hiromi Sesaki |
| 概要 | Cardiolipin, a unique phospholipid composed of four fatty acid chains, is located mainly in the mitochondrial inner membrane (IM). Cardiolipin is required for the integrity of several protein complexes in the IM, including the TIM23 translocase, a dynamic complex which mediates protein import into the mitochondria through interactions with the import motor presequence translocase-associated motor (PAM). In this study, we report that two homologous intermembrane space proteins, Ups1p and Ups2p, control cardiolipin metabolism and affect the assembly state of TIM23 and its association with PAM in an opposing manner. In ups1 Delta mitochondria, cardiolipin levels were decreased, and the TIM23 translocase showed altered conformation and decreased association with PAM, leading to defects in mitochondrial protein import. Strikingly, loss of Ups2p restored normal cardiolipin levels and rescued TIM23 defects in ups1 Delta mitochondria. Furthermore, we observed synthetic growth defects in ups mutants in combination with loss of Pam17p, which controls the integrity of PAM. Our findings provide a novel molecular mechanism for the regulation of cardiolipin metabolism. |
| DOI | 10.1083/jcb.200812018 |
| ISSN | 0021-9525 |