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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1996/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF BIOLOGICAL CHEMISTRY |
| 出版社・発行元 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 巻・号・頁 | 271(17),pp.9875-9878 |
| 著者・共著者 | N Itano,K Kimata |
| 概要 | We developed a mammalian transient expression system to isolate cDNA clones that determine hyaluronan expression. HAS(-), a mouse mammary carcinoma mutant cell line, which is defective in hyaluronan synthase activity, was first established and used as a recipient for the expression cloning, One cloned cDNA that overcame the deficiency was isolated, The cDNA termed HAS contains an open reading frame of 1749 base pairs encoding a new protein of 583 amino acids, Homology analysis of the amino acid sequence suggests that HAS protein is related to streptococcal hyaluronan synthase and also to Xenopus laevis DG42 protein that was found to be homologous to bacterial hyaluronan synthase. Expression of HAS cDNA in HAS(-) cells complemented not only their mutant phenotypes such as deficient hyaluronan-matrix deposition but also hyaluronan synthase activity itself, Therefore, HAS cDNA is responsible for the activity of the hyaluronan synthase, a key enzyme of hyaluronan synthesis in eukaryotic cells. |
| ISSN | 0021-9258 |