|
ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2022/07 |
| 形態種別 | 研究論文 |
| 標題 | Structures of Multisubunit Membrane Complexes With the CRYO ARM 200. |
| 執筆形態 | その他 |
| 掲載誌名 | Microscopy (Oxford, England) |
| 掲載区分 | 国外 |
| 巻・号・頁 | 71(5),pp.249-261 |
| 著者・共著者 | Christoph Gerle,Jun-Ichi Kishikawa,Tomoko Yamaguchi,Atsuko Nakanishi,Orkun Çoruh,Fumiaki Makino,Tomoko Miyata,Akihiro Kawamoto,Ken Yokoyama,Keiichi Namba,Genji Kurisu,Takayuki Kato |
| 概要 | Progress in structural membrane biology has been significantly accelerated by the ongoing 'Resolution Revolution' in cryo electron microscopy (cryo-EM). In particular, structure determination by single particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor LP-ring from Salmonella enterica. |
| DOI | 10.1093/jmicro/dfac037 |
| PMID | 35861182 |