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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2021/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana. |
| 執筆形態 | その他 |
| 掲載誌名 | Proceedings of the National Academy of Sciences of the United States of America |
| 掲載区分 | 国外 |
| 巻・号・頁 | 118(51) |
| 著者・共著者 | Yuichi Yokochi,Yuka Fukushi,Ken-Ichi Wakabayashi,Keisuke Yoshida,Toru Hisabori |
| 概要 | Thioredoxin (Trx) is a protein that mediates the reducing power transfer from the photosynthetic electron transport system to target enzymes in chloroplasts and regulates their activities. Redox regulation governed by Trx is a system that is central to the adaptation of various chloroplast functions to the ever-changing light environment. However, the factors involved in the opposite reaction (i.e., the oxidation of various enzymes) have yet to be revealed. Recently, it has been suggested that Trx and Trx-like proteins could oxidize Trx-targeted proteins in vitro. To elucidate the in vivo function of these proteins as oxidation factors, we generated mutant plant lines deficient in Trx or Trx-like proteins and studied how the proteins are involved in oxidative regulation in chloroplasts. We found that f-type Trx and two types of Trx-like proteins, Trx-like 2 and atypical Cys His-rich Trx (ACHT), seemed to serve as oxidation factors for Trx-targeted proteins, such as fructose-1,6-bisphosphatase, Rubisco activase, and the γ-subunit of ATP synthase. In addition, ACHT was found to be involved in regulating nonphotochemical quenching, which is the mechanism underlying the thermal dissipation of excess light energy. Overall, these results indicate that Trx and Trx-like proteins regulate chloroplast functions in concert by controlling the redox state of various photosynthesis-related proteins in vivo. |
| DOI | 10.1073/pnas.2114952118 |
| PMID | 34907017 |