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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2002/08 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Crystallization and preliminary X-ray diffraction analysis of glutamate dehydrogenase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 |
| 執筆形態 | その他 |
| 掲載誌名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY |
| 出版社・発行元 | BLACKWELL MUNKSGAARD |
| 巻・号・頁 | 58,pp.1338-1339 |
| 著者・共著者 | MW Bhuiya,H Tsuge,SK Yoneda,N Katunuma,T Ohshima |
| 概要 | Glutamate dehydrogenase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1, was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol (PEG) 400 as the precipitant. The crystals belong to the hexagonal space group P6(3), with unit-cell parameters a = b = 98.9, c = 394.8 Angstrom, alpha = beta = 90, gamma = 120degrees. The asymmetric unit contained one hexamer of the enzyme, giving a crystal volume per enzyme mass (V-M) of 1.98 Angstrom(3) Da(-1) and a solvent content of 37.3%. The X-ray diffraction data were collected to a resolution of 3.0 Angstrom at the BL6B beamline in the Photon Factory with an overall R-sym of 13.8% and a completeness of 87.1%. |
| DOI | 10.1107/S0907444902008478 |
| ISSN | 0907-4449 |