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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2023/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Cells collectively migrate during ammonium chemotaxis in Chlamydomonas reinhardtii. |
| 執筆形態 | その他 |
| 掲載誌名 | Scientific reports |
| 掲載区分 | 国外 |
| 巻・号・頁 | 13(1),pp.10781-10781 |
| 著者・共著者 | Gabela Nelson,Alexis Strain,Atsuko Isu,Alireza Rahnama,Ken-Ichi Wakabayashi,Adam T Melvin,Naohiro Kato |
| 概要 | The mechanisms governing chemotaxis in Chlamydomonas reinhardtii are largely unknown compared to those regulating phototaxis despite equal importance on the migratory response in the ciliated microalga. To study chemotaxis, we made a simple modification to a conventional Petri dish assay. Using the assay, a novel mechanism governing Chlamydomonas ammonium chemotaxis was revealed. First, we found that light exposure enhances the chemotactic response of wild-type Chlamydomonas strains, yet phototaxis-incompetent mutant strains, eye3-2 and ptx1, exhibit normal chemotaxis. This suggests that Chlamydomonas transduces the light signal pathway in chemotaxis differently from that in phototaxis. Second, we found that Chlamydomonas collectively migrate during chemotaxis but not phototaxis. Collective migration during chemotaxis is not clearly observed when the assay is conducted in the dark. Third, the Chlamydomonas strain CC-124 carrying agg1-, the AGGREGATE1 gene (AGG1) null mutation, exhibited a more robust collective migratory response than strains carrying the wild-type AGG1 gene. The expression of a recombinant AGG1 protein in the CC-124 strain suppressed this collective migration during chemotaxis. Altogether, these findings suggest a unique mechanism; ammonium chemotaxis in Chlamydomonas is mainly driven by collective cell migration. Furthermore, it is proposed that collective migration is enhanced by light and suppressed by the AGG1 protein. |
| DOI | 10.1038/s41598-023-36818-6 |
| PMID | 37402785 |