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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2022/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The mammalian-type thioredoxin reductase 1 confers a high-light tolerance to the green alga Chlamydomonas reinhardtii. |
| 執筆形態 | その他 |
| 掲載誌名 | Biochemical and biophysical research communications |
| 掲載区分 | 国外 |
| 巻・号・頁 | 596,pp.97-103 |
| 担当区分 | 責任著者 |
| 著者・共著者 | Yuma Asahina,Kazuma Sakamoto,Toru Hisabori,Ken-Ichi Wakabayashi |
| 概要 | Reactive oxygen species (ROS) can both act as a poison causing cell death and important signaling molecules among various organisms. Photosynthetic organisms inevitably produce ROS, making the appropriate elimination of ROS an essential strategy for survival. Interestingly, the unicellular green alga Chlamydomonas reinhardtii expresses a mammalian form of thioredoxin reductase, TR1, which functions as a ROS scavenger in animal cells. To investigate the properties of TR1 in C. reinhardtii, we generated TR1 knockout strains using CRISPR/Cas9-based genome editing. We found a reduced tolerance to high-light and ROS stresses in the TR1 knockout strains compared to the parental strain. In addition, the regulation of phototactic orientation, known to be regulated by ROS, was affected in the knockout strains. These results suggest that TR1 contributes to a ROS-scavenging pathway in C. reinhardtii. |
| DOI | 10.1016/j.bbrc.2022.01.088 |
| PMID | 35121375 |