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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2004/11 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Crystallization and preliminary X-ray diffraction analysis of homing endonuclease I-Tsp061I |
| 執筆形態 | その他 |
| 掲載誌名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY |
| 出版社・発行元 | BLACKWELL MUNKSGAARD |
| 巻・号・頁 | 60(60(11)),pp.2006-2008 |
| 担当区分 | 最終著者,責任著者 |
| 著者・共著者 | T Imagawa,H Nakayama,N Katunuma,H Sakuraba,T Ohshima,T Itoh,Y Sako,N Nomura,H Tsuge |
| 概要 | Two crystal forms, rhombohedral and hexagonal, of a homing endonuclease from Thermoproteus sp. IC-061 (I-Tsp0611) were obtained by the hanging-drop and sitting-drop method, respectively. The hexagonal crystals belong to space group P6(3)22, with unit-cell parameters a = b = 111.4, c = 97.6 Angstrom, and diffract to 3.2 Angstrom resolution on beamline BL44 at SPring-8 (Harima, Japan). The rhombohedral crystals belong to space group R32, with unit-cell parameters a = b = 95.4, c = 192.9 Angstrom, and diffract to 2.7 Angstrom resolution using a Cu Kalpha rotating-anode generator with an R-AXIS VII detector. The crystal asymmetric unit contained one protein molecule and the solvent contents of the two crystal forms were estimated to be 68.3 and 67.6% by volume, respectively. |
| DOI | 10.1107/S0907444904020232 |
| ISSN | 0907-4449 |