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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2011/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Dual role of the receptor Tom20 in specificity and efficiency of protein import into mitochondria |
| 執筆形態 | その他 |
| 掲載誌名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
| 出版社・発行元 | NATL ACAD SCIENCES |
| 巻・号・頁 | 108(1),pp.91-96 |
| 著者・共著者 | Hayashi Yamamoto,Nobuka Itoh,Shin Kawano,Yoh-ichi Yatsukawa,Takaki Momose,Tadashi Makio,Mayumi Matsunaga,Mihoko Yokota,Masatoshi Esaki,Toshihiro Shodai,Daisuke Kohda,Alyson E. Aiken Hobbs,Robert E. Jensen,Toshiya Endo |
| 概要 | Mitochondria import most of their resident proteins from the cytosol, and the import receptor Tom20 of the outer-membrane translocator TOM40 complex plays an essential role in specificity of mitochondrial protein import. Here we analyzed the effects of Tom20 binding on NMR spectra of a long mitochondrial presequence and found that it contains two distinct Tom20-binding elements. In vitro import and cross-linking experiments revealed that, although the N-terminal Tom20-binding element is essential for targeting to mitochondria, the C-terminal element increases efficiency of protein import in the step prior to translocation across the inner membrane. Therefore Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency. |
| DOI | 10.1073/pnas.1014918108 |
| ISSN | 0027-8424 |