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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2011/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | TIM23-mediated insertion of transmembrane alpha-helices into the mitochondrial inner membrane |
| 執筆形態 | その他 |
| 掲載誌名 | EMBO JOURNAL |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 30(6),pp.1003-1011 |
| 著者・共著者 | Salome Calado Botelho,Marie Osterberg,Andreas S. Reichert,Koji Yamano,Patrik Bjorkholm,Toshiya Endo,Gunnar von Heijne,Hyun Kim |
| 概要 | While overall hydrophobicity is generally recognized as the main characteristic of transmembrane (TM) alpha-helices, the only membrane system for which there are detailed quantitative data on how different amino acids contribute to the overall efficiency of membrane insertion is the endoplasmic reticulum (ER) of eukaryotic cells. Here, we provide comparable data for TIM23-mediated membrane protein insertion into the inner mitochondrial membrane of yeast cells. We find that hydrophobicity and the location of polar and aromatic residues are strong determinants of membrane insertion. These results parallel what has been found previously for the ER. However, we see striking differences between the effects elicited by charged residues flanking the TM segments when comparing the mitochondrial inner membrane and the ER, pointing to an unanticipated difference between the two insertion systems. The EMBO Journal (2011) 30, 1003-1011. doi:10.1038/emboj.2011.29; Published online 15 February 2011 |
| DOI | 10.1038/emboj.2011.29 |
| ISSN | 0261-4189 |