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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1994/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | MICROMACHINED ELECTROPORATION SYSTEM FOR TRANSGENIC FISH |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF BIOTECHNOLOGY |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 34(1),pp.35-42 |
| 著者・共著者 | Y MURAKAMI,K MOTOHASHI,K YANO,K IKEBUKURO,K YOKOYAMA,E TAMIYA,KARUBE, I |
| 概要 | Luciferase gene was introduced into fertilized eggs of medaka by a localized electric field between thin film electrodes formed on a glass plate. Miniaturization of the electrodes enabled us to apply a localized electric field to the animal pole of the fertilized egg. Biochemical luminescence and an electrophoresis pattern showed expression and integration of the gene, respectively. The presented system had a higher ratio of gene introduction than the conventional electroporation method. |
| DOI | 10.1016/0168-1656(94)90163-5 |
| ISSN | 0168-1656 |
| NAID | 80007602512 |
| PMID | 7764743 |