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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2018/10 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Deadenylation by the CCR4-NOT complex contributes to the turnover of hairy-related mRNAs in the zebrafish segmentation clock. |
| 執筆形態 | その他 |
| 掲載誌名 | FEBS Letters |
| 掲載区分 | 国外 |
| 巻・号・頁 | 592(20),pp.3388-3398 |
| 著者・共著者 | Fujino Y,Yamada K,Sugaya C,Ooka Y,Ovara H,Ban H,Akama K,Otosaka S,Kinoshita H,Yamasu K,Mishima Y,Kawamura A |
| 概要 | In the zebrafish segmentation clock, hairy/enhancer of split-related genes her1, her7, and hes6 encodes components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm (PSM), these hairy-related mRNAs are subject to strict post-transcriptional regulation. In this study, we demonstrate that inhibition of the CCR4-NOT deadenylase complex lengthens poly(A) tails of hairy-related mRNAs and increases the amount of these mRNAs, which is accompanied by defective somite segmentation. In transgenic embryos, we show that EGFP mRNAs with 3'UTRs of hairy-related genes exhibit turnover similar to endogenous mRNAs. Our results suggest that turnover rates of her1, her7, and hes6 mRNAs are differently regulated by the CCR4-NOT deadenylase complex possibly through their 3'UTRs in the zebrafish PSM. |
| DOI | 10.1002/1873-3468.13261 |
| PMID | 30281784 |