ミシマ ユウイチロウ   Mishima Yuichiro
  三嶋 雄一郎
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2018/10
形態種別 研究論文
査読 査読あり
標題 Deadenylation by the CCR4-NOT complex contributes to the turnover of hairy-related mRNAs in the zebrafish segmentation clock.
執筆形態 その他
掲載誌名 FEBS Letters
掲載区分国外
巻・号・頁 592(20),pp.3388-3398
著者・共著者 Fujino Y,Yamada K,Sugaya C,Ooka Y,Ovara H,Ban H,Akama K,Otosaka S,Kinoshita H,Yamasu K,Mishima Y,Kawamura A
概要 In the zebrafish segmentation clock, hairy/enhancer of split-related genes her1, her7, and hes6 encodes components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm (PSM), these hairy-related mRNAs are subject to strict post-transcriptional regulation. In this study, we demonstrate that inhibition of the CCR4-NOT deadenylase complex lengthens poly(A) tails of hairy-related mRNAs and increases the amount of these mRNAs, which is accompanied by defective somite segmentation. In transgenic embryos, we show that EGFP mRNAs with 3'UTRs of hairy-related genes exhibit turnover similar to endogenous mRNAs. Our results suggest that turnover rates of her1, her7, and hes6 mRNAs are differently regulated by the CCR4-NOT deadenylase complex possibly through their 3'UTRs in the zebrafish PSM.
DOI 10.1002/1873-3468.13261
PMID 30281784