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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1998/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | A novel approach to crystallizing proteins with temperature-induction method: GrpE protein from Thermus thermophilus |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF CRYSTAL GROWTH |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 186(3),pp.456-460 |
| 著者・共著者 | K Kitano,K Motohashi,M Yoshida,K Miki |
| 概要 | GrpE protein from Thermus thermophilus has been crystallized by the use of both 2-methyl-2,4-pentanediol and acetic acid as precipitants. It was found that heat treatment at 40 degrees C was indispensable to obtain single crystals of this protein. Here we report one of the most typical examples of the effective temperature induction method in protein crystallization. In this case, no crystals were obtained if the heat treatment was not employed. (C) 1998 Elsevier Science B.V. All rights reserved. |
| DOI | 10.1016/S0022-0248(97)00513-7 |
| ISSN | 0022-0248 |
| NAID | 80010217482 |