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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/09 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Roles of Dom34:Hbs1 in Nonstop Protein Clearance from Translocators for Normal Organelle Protein Influx |
| 執筆形態 | その他 |
| 掲載誌名 | CELL REPORTS |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 2(3),pp.447-453 |
| 著者・共著者 | Toshiaki Izawa,Tatsuhisa Tsuboi,Kazushige Kuroha,Toshifumi Inada,Shuh-ichi Nishikawa,Toshiya Endo |
| 概要 | Because messenger RNAs without a stop codon (nonstop mRNAs) generate stalled ribosomes, cells have developed a mechanism allowing degradation of nonstop mRNAs and their translation products (nonstop proteins) in the cytosol. Here, we observe the fate of nonstop proteins destined for organelles such as the endoplasmic reticulum (ER) and mitochondria. Nonstop mRNAs for secretory-pathway proteins in yeast generate nonstop proteins that become stuck in the translocator, the Sec61 complex, in the ER membrane. These stuck nonstop secretory proteins avoid proteasomal degradation in the cytosol, but are instead released into the ER lumen through stalled ribosome and translocator channels by Dom34:Hbs1. We also found that nonstop mitochondrial proteins are cleared from the mitochondrial translocator, the TOM40 complex, by Dom34:Hbs1. Clearance of stuck nonstop proteins from organellar translocator channels is crucial for normal protein influx into organelles and for normal cell growth, especially when nonstop mRNA decay does not function efficiently. |
| DOI | 10.1016/j.celrep.2012.08.010 |
| ISSN | 2211-1247 |