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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2002/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The outer dynein arm-docking complex: Composition and characterization of a subunit (Oda1) necessary for outer arm assembly |
| 執筆形態 | その他 |
| 掲載誌名 | MOLECULAR BIOLOGY OF THE CELL |
| 出版社・発行元 | AMER SOC CELL BIOLOGY |
| 巻・号・頁 | 13(3),pp.1015-1029 |
| 著者・共著者 | S Takada,CG Wilkerson,K Wakabayashi,R Kamiya,GB Witman |
| 概要 | To learn more about how dyneins are targeted to specific sites in the flagellum, we have investigated a factor necessary for binding of outer arm dynein to the axonemal microtubules of Chlamydomonas. This factor, termed the outer dynein arm-docking complex (ODA-DC), previously was shown to be missing from axonemes of the outer dynein armless mutants oda1 and oda3. We have now partially purified the ODA-DC, determined that it contains equimolar amounts of M-r similar to105,000 and similar to70,000 proteins plus a third protein of M-r similar to25,000, and found that it is associated with the isolated outer arm in a 1:1 molar ratio. We have cloned a full-length cDNA encoding the M-r similar to70,000 protein; the sequence predicts a 62.5-kDa protein with potential homologs in higher ciliated organisms, including humans. Sequencing of corresponding cDNA from strain odal revealed it has a mutation resulting in a stop codon just downstream of the initiator ATG; thus, it is unable to make the full-length M-r similar to70,000 protein. These results demonstrate that the ODA1 gene encodes the M-r similar to70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm onto the doublet microtubule. |
| DOI | 10.1091/mbc.01-04-0201 |
| ISSN | 1059-1524 |
| PMID | 11907279 |