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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2013 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Analyses of protein-protein interactions by in vivo photocrosslinking in budding yeast |
| 執筆形態 | その他 |
| 掲載誌名 | Methods in Molecular Biology |
| 巻・号・頁 | 1033,pp.207-217 |
| 著者・共著者 | Takuya Shiota,Shuh-Ichi Nishikawa,Toshiya Endo |
| 概要 | Recent development of methods for genetic incorporation of unnatural amino acids into proteins in live cells enables us to analyze protein interactions by site-specific photocrosslinking. Here we describe a method to incorporate p-benzoyl-l-phenylalanine (pBpa), a photoreactive unnatural amino acid, into defined positions of a target protein in living yeast cells. Photocrosslinking using the pBpa-incorporated proteins has been proven to be a powerful method for analyzing protein-protein interactions at the spatial resolution of amino-acid residues. Since photocrosslinking can be performed for pBpa-incorporated proteins that are properly assembled into a protein complex in living cells, this method will allow us to reveal protein-protein interactions of the target proteins at work. © 2013 Springer Science+Business Media, LLC. |
| DOI | 10.1007/978-1-62703-487-6_14 |
| ISSN | 1064-3745 |
| PMID | 23996180 |