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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2020/12 |
| 形態種別 | 研究論文 |
| 標題 | Organelle membrane-specific chemical labeling and dynamic imaging in living cells. |
| 執筆形態 | その他 |
| 掲載誌名 | Nature chemical biology |
| 掲載区分 | 国外 |
| 巻・号・頁 | 16(12),pp.1361-1367 |
| 著者・共著者 | Tomonori Tamura,Alma Fujisawa,Masaki Tsuchiya,Yuying Shen,Kohjiro Nagao,Shin Kawano,Yasushi Tamura,Toshiya Endo,Masato Umeda,Itaru Hamachi |
| 概要 | Lipids play crucial roles as structural elements, signaling molecules and material transporters in cells. However, the functions and dynamics of lipids within cells remain unclear because of a lack of methods to selectively label lipids in specific organelles and trace their movement by live-cell imaging. We describe here a technology for the selective labeling and fluorescence imaging (microscopic or nanoscopic) of phosphatidylcholine in target organelles. This approach involves the metabolic incorporation of azido-choline, followed by a spatially limited bioorthogonal reaction that enables the visualization and quantitative analysis of interorganelle lipid transport in live cells. More importantly, with live-cell imaging, we obtained direct evidence that the autophagosomal membrane originates from the endoplasmic reticulum. This method is simple and robust and is thus powerful for real-time tracing of interorganelle lipid trafficking. |
| DOI | 10.1038/s41589-020-00651-z |
| PMID | 32958953 |