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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2000/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Evidence for the presence of an F-type ATP synthase involved in sulfate respiration in Desulfovibrio vulgaris |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF BACTERIOLOGY |
| 出版社・発行元 | AMER SOC MICROBIOLOGY |
| 巻・号・頁 | 182(8),pp.2200-2206 |
| 著者・共著者 | K Ozawa,T Meikari,K Motohashi,M Yoshida,H Akutsu |
| 概要 | Using a library of genomic DNA from Desulfovibrio vulgaris Miyazaki F, a strict anaerobe, and two synthetic deoxyoligonucleotide probes designed for F-type ATPases, the genes for open reading frames (ORFs) 1 to 5 were cloned and sequenced. The predicted protein sequences of the gene products indicate that they are composed of 172, 488, 294, 471, and 134 amino acids, respectively, and that they share considerable identity at the amino acid level with delta, alpha, gamma, beta, and epsilon subunits found in other F-type ATPases, respectively. Furthermore, a component carrying ATPase activity was partially purified from the cytoplasmic membrane fraction of the D. vulgaris Miyazaki F cells. The N-terminal amino acid sequences of three major polypeptides separated by sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis were identical to those of the products predicted by the sequences of ORF-2, ORF-3, and ORF-4, suggesting that an F-type ATPase is functioning in the D. vulgaris Miyazaki F cytoplasmic membrane. The amount of the F-type ATPase produced in the D. vulgaris Miyazaki F cells is similar to that in the Escherichia coli cells cultured aerobically. It indicates that the enzyme works as an ATP synthase in the D. vulgaris Miyazaki F cells in connection with sulfate respiration. |
| DOI | 10.1128/JB.182.8.2200-2206.2000 |
| ISSN | 0021-9193 |
| NAID | 80011673896 |
| PMID | 10735863 |