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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1991 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Purification by dye-ligand chromatography and a crystallization study of the F1-ATPase and its major subunits, β and α, from a thermophilic bacterium, PS3 |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Biochemistry |
| 出版社・発行元 | Oxford University Press |
| 巻・号・頁 | 109(3),pp.466-471 |
| 著者・共著者 | Yasuo Shirakihara,Masafumi Yohda,Yasuo Kagawa,Ken Yokoyama,Masasuke Yoshida |
| 概要 | For a crystallization study, purification methods for F1i-ATPase from a thermophilic bacterium, PS3, and its major subunits, β and α, have been improved. The improvement depended on the introduction of dye-ligand chromatography columns to the previously adopted array of chromatography columns: a Blue-B (a blue dye bound to agarose) column was introduced for the F1, preparation, a Green-A column (a green dye attached to agarose) for the β subunit, and a Blue-A (another blue dye, Cibacron Blue 3GA, bound to agarose) column for the α subunit. The improved preparations of all the proteins had purities of nearly 99%. Using the highly purified preparations of the proteins, crystallization conditions were searched for in a systematic way. Large plate crystals (0.2×0.5×0.5 mm) of Fi were grown from a polyethylene glycol solution. However, neither of the subunits was crystallized, in spite of extensive search for crystallization conditions. © 1991 by The Journal of Biochemistry. |
| DOI | 10.1093/oxfordjournals.jbchem.a123405 |
| ISSN | 0021-924X |
| PMID | 1831812 |