|
ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 1995 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Transcriptional regulation of the vimentin-encoding gene in mouse myeloid leukemia M1 cells |
| 執筆形態 | その他 |
| 掲載誌名 | Gene |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 166(2),pp.281-286 |
| 著者・共著者 | Nakamura, N.,Shida, M.,Hirayoshi, K.,Nagata, K. |
| 概要 | To investigate the regulatory mechanisms controlling expression of the vimentin-encoding gene (Vim) during mouse myeloid leukemia M1 cell differentiation, mouse Vim was cloned and the transcriptional activity of its 5' promoter region was analysed by chloramphenicol acetyltransferase (CAT) assay. Analyses of various deletion mutants revealed that a 188-bp fragment of the proximal Vim promoter (pVim) was sufficient for effective transcription in M1 cells. This 188-bp sequence is highly conserved between mouse, hamster and human. Further deletion analyses revealed that a minimum promoter element (-44 to +26) is essential for basic promoter function and could respond to cell differentiation. Detailed analyses of mutant and chimeric pVim constructs defined a CCAAT box at -89 to -84 to be an essential positive regulatory element. A G + C-rich element between the CCAAT and TATA boxes was found to act as a strong negative regulatory element in Vim transcription. |
| DOI | 10.1016/0378-1119(95)00600-1 |
| ISSN | 0378-1119 |
| Put Code(ORCID) | 19809427 |